Antihypertensive and anticoagulant properties of glycosaminoglycans extracted from the sturgeon (Acipenser persicus) cartilage
Curr Issues Pharm Med Sci., Vol. 31, No. 4, 163-169
Katayoon Karimzadeh
Department of Marine Biology, Lahijan Branch, Islamic Azad University, Lahijan, Iran
10.1515/cipms-2018-0031
© 2018 Author(s). This is an open access article distributed under the Creative Commons Attribution-NonComercial-No Derivs licence (http://creativecommons.org/licenses/by-nc-nd/3.0/)
Abstract
Large amounts of valuable waste are produced during sea food processing. This has a great potential for conversion to biologically active proteins and polysaccharides. Among these compounds, sulfated polysaccharides have been considered due to their many biological properties.
The present work was conducted to study anticoagulant activities and angiotensin-I converting enzyme (ACE) inhibitory effects of glycosaminoglycans (GAGs) extracted from the cartilage of sturgeon (Acipenser persicus). The enzymatic extraction of sturgeon cartilage was performed in the presence of cetylpyridinium chloride salt. The structure was characterized via electron microscope and Fourier transform infrared spectroscopy (FTIR) analysis. Herein, ACE inhibitory and anticoagulant properties of extracted GAGs were determined.
The amount of GAGs was 6.8±1.3% of cartilage dry weight. GAGs showed good activity in ACE inhibitory – with a highest level of 85.7%. The derived anticoagulant activity indexes, APPT (activated partial thromboplastin time) and TT (Thrombin time) of the extracted polysaccharide showed a prolonging of clotting time, compare to control. The results of this study revealed that the cartilage extracted GAGs possess promising ACE inhibitory properties and anticoagulant effects. Thus, the product can be substituted for blood reducing drugs and antithrombotic agents at least in laboratory conditions.
Full Text
Keywords
sturgeon, cartilage, angiotensin I-converting enzyme, anticoagulant activity, alcalase.
References
- Ambigaipalan P, Shahidi F. Bioactive peptides from shrimp shell processing discards: Antioxidant and biological activities. J Funct Foods.2017;34:7-17.
- Krichen F, Karaoud W, Sayari N, Sila A, Kallel F, Ellouz-Chaabouni S, Bougatef A. Sulfated Polysaccharides from Tunisian Fish Skins: Antioxidant, DNA Damage Protective Effect and Antihypertensive Activities. J Polym Environ.2016;24(2):166-75.
- FAO. The State of World Fisheries and Aquaculture 2016. Contributing to food security and nutrition for all. Rome. 200 pp.
- Ely R, Supriya T, Naik C. Antimicrobial activity of marine organisms collected off the coast of South East India. J Exp Mar Bio Ecol.2004;309:121-7.
- Sugahara K, Mikami T, Uyama T, et al. Recent advances in the structural biology of chondroitin sulfate and dermatan sulfate. Curr Opin Struct Biol. 2003;13:612-20.
- Zhang F, Xie J, Linhardt R.J. Isolation and structural characterization of glycosaminoglycans from heads of red salmon (Oncorhynchus nerka). Biotechnol Bioeng.2014;1(1):002.
- Wang Z, Zhao Y, Su T. Extraction and antioxidant activity of polysaccharides from Rana chensinensis skin. Carbohydr Polym. 2015;115:25-31.
- Yang J, Wanga Y, Jiang T, Lv Z. Novel branch patterns and anticoagulant activity of glycosaminoglycan from sea cucumber Apostichopus japonicus. Int JBiol Macromol.2015;72:911-8.
- Rajapakse N, Jung WK, Mendis E, Moon SH, Kim SK. A novel anticoagulant purified from fish protein hydrolysate inibitis factor XIIa and platelet aggregation. Life Science. 2005;76(22): 2607-19.
- Krichen F, Karaoud W, Sila A, Abdelmalek BE, Ghorbel R, Ellouz-Chaabouni S, Bougatef A. Extraction, characterization and antimicrobial activity of sulfated polysaccharides from fish skins. Int JBiol Macromol. 2015;75:283-9.
- Ben Mansour H. Majdoub H, Bataille I, Roudesli M.S, Hassine M, Ajzenberg N. et al., Polysaccharides from the skin of the ray Raja radula. Partial characterization and anticoagulant activity. Thromb Res. 2009;123: 671-8.
- Udenigwe CC, Lin Y-S, Hou W-C, et al. Kinetics of the inhibition of renin and angiotensin I-converting enzyme by flaxseed protein hydro lysate fractions. J Funct Foods. 2009;1:199-207.
- Kijjoa A, Sawangwong P. Drugs and cosmetics from the sea. Mar Drugs. 2004;2:73-82.
- Choonpicharn S, Jaturasitha S, Rakariyatham N, et al. Antioxidant and antihypertensive activity of gelatin hydrolysate from Nile tilapia skin. J Food Sci Technol. 2015;52:3134-9.
- Ye H, Wang KQ, Zhou CH, Liu J, Zeng XX. Purification, antitumor and antioxidant activities in vitro of polysaccharides from the brown seaweed Sargassum pallidum. Food Chem. 2008;111:428.
- Souza BWS, Cerqueira MA, Bourbon A I, Pinheiro AC, Martins JT, Teixeira JA. et al. Chemical characterization and antioxidant activity of sulfated polysaccharide from the red seaweed Gracilaria birdiae, Food Hydrocoll. 2012;27:287-92.
- Yamada S, Sugahara K, Özbek S. Evolution of glycosamino-glycans: comparative biochemical study. Commun Integr Biol 2011;4:150-8.
- Yamada S, Sugahara K. Potential therapeutic application of chondroitin sulfate/dermatan sulfate. Current Drug Discovery Tech. 2008;5:289-301.
- Delshad S, Karimzadeh K, Mostafaie A. Extraction of Chondroitin Sulfate From Cartilage Sturgeon, Stellate Sturgeon (Acipenser stellatus) and its Inductive Effect on Human Fibroblast Proliferation. Iran South Med J. 2017;20(4):349-61.
- Maccari F, Ferrarini F, Volpi N. Structural characterization of chondroitin sulfate from sturgeon bone. Carbohydr Res. 2010;345(11):1575-80.
- Farndale WR, Buttle, DJ, Barrett, A J. Improved quantitation and discrimination of sulphated glycosaminoglycans by use of dimethylmethylene blue, Biochim Biophys Acta. 1986;883173-7.
- Nakamura Y, Yamamoto N, Sakai K, Okubo A, Yamazaki S, Takano T. Purification and characterization of angiotensin I-converting-enzyme inhibitors from Sour milk. J Dairy Sci. 1996;78:777.
- Tingbø MG, Kolset SO, Ofstad R, Enersen G, Hannesson KO.Sulfated glycosaminoglycans in the extracellular matrix of muscle tissue in Atlantic cod (Gadus morhua) and Spotted wolfish (Anarhichas minor). Comp Biochem Physiol B Biochem Mol Biol. 2005;140:349-57.
- Shahidi F, Ambigaipalan P. Novel functional food ingredients from marine sources. Curr Opin Food Sci. 2015;2:123-9. 28.
- Krylov VB, Grachev AA, Ustyuzhanina NE, Ushakova NA, Preobrazhenskaya ME, et al. Preliminary structural characterization, anti-inflammatory and anticoagulant activities of chondroitin sulfates from marine fish cartilage. Russ Chem Bull. 2011;60(4): 746-53.
- Garnjanagoonchorn W, Wongekalak L, Engkagul A. Determination of chondroitin sulfate from different sources of cartilage. Chem Eng Prog. 2007;46:465-71.
- Pan Y, Wang P, Zahang F, Lin L, Linhardt RJ. Glycosaminoglycans from fish swim bladder: isolation, structural characterization and bioactive potential. Glycoconj J. 2017. doi: 10.1007/s10719-017-9804-5. [PubMed: 29124565].
- Abdelmalek BE, Sila A, Krichen F, Karoud W, Martinez-Alvarez O, Ellouz-Chaabouni S, Ayadi MA, Bougatef A. Sulfated polysaccharides from Loligo vulgaris skin: potential biological activities and partial purification. Int J Biol Macromol. 2015;72:2015, 72:1143-51.
- Chen Y, Zhang H, Wang Y, Nie S, Li C, Xie M. Acetylation and carboxymethylation of the polysaccharide from Ganoderma atrum and their antioxidant and immunomodulating activities. Food Chem. 2014;156:279-88.
- Cardozo FTGS, Camelini CM, Cordeiro MNS, Mascarello A, Malagoli BG, Larsen IV, Rossi MJ, Nunes RJ, Braga FC, Brandt CR, Simo˜es CMO. Characterization and cytotoxic activity of sulfated derivatives of polysaccharides from Agaricus brasiliensis. Int J Biol Macromol. 2013;57:265-72.
- Hong JH, Choi YH Physico-chemical properties of protein-bound polysaccharide from Agaricus blazei Murill prepared by ultrafiltration and spray drying process. Int J Food Sci Technol. 2006;42(1):1-8.
- Balti R, Nedjar-Arroume N, Bougatef A, Guillochon D, Nasri M Three novel angiotensin I-converting enzyme (ACE) inhibitory peptides from cuttlefish (Sepia officinalis) using digestive proteases. Food Res Int. 2010;43:11361-1143.
- Bougatef A, Nedjar-Arroume N, Ravallec-Ple´ R, Leroy Y, Guillochon D, Barkia A, Nasri M Angiotensin I-converting enzyme (ACE) inhibitory activities of sardinelle (Sardinella aurita) by-products protein hydrolysates obtained by treatment with microbial and visceral fish serine proteases. Food Chem. 2008;111:350-6.
- Sesoko S, Kaneko Y. Cough associated with the use of Captopril. Arch Intern Med.1985;145(8):1524-1524.
- Wang Y. Zhao, T. Su. Extraction and antioxidant activity of polysaccharides from Rana chensinensis skin. Carbohydr Polym. 2015;115:25-31.
- Gui M, Song J, Zhang L, Wang S, Ma C, Li P. Chemical characteristics and antithrombotic effect of chondroitin sulfates from sturgeon skull and sturgeon backbone. Carbohydrate Polymers. 2015;http://dx.doi.org/10.1016/j.carbpol.2015.01.046.
- Majdoub H, Mansour MB, Chaubet F, Roudesli MS, Maaroufi RM. Anticoagulant activity of a sulfated polysaccharide from the green alga Arthrospira platensis. Biochem BiophActa (BBA) - General Subjects. 2009;1790(10):1377-81.
- Shute J. Glycosaminoglycan and chemokine/growth factor interactions. Handb. Exp. Pharmacol.2012;207:307-24.
